UIDL provides a full range of Dermatopathology consultation services. Immunofluorescence is a technique used on fresh tissue or tissue placed in special media. This technique provides additional information that cannot be obtained by usual fixation and biopsy processing. This procedure describes how specimens are sent to UIDL for immunofluorescence.
1. The specimen is received in the Mercy Anatomic Pathology (AP) gross room and assigned a case number in CoPath.
A. The part type for immunofluorescence is “SKIMM”.
B. Gross description is performed by the Pathology Assistant.
C. Place the punch biopsy in a specimen bottle containing Michel’s transport media.
Note: Most specimens come from Dr. Jennifer Schope’s office and are already in Michel’s media. UCL supplies Dr. Schope’s office with Michel’s media as needed.
a. The Michel’s media is stored in the gross room refrigerator.
b. Michel’s transport media must be refrigerated and is stable for 6-8 months. If the media becomes cloudy it must not be used.
c. Both Mercy and Finley gross rooms are kept stocked with a current supply.
2. A completed “UIDL Anatomic Pathology Consult” requisition is required for all specimens.
A. Complete all applicable fields including: clinical history, insurance and policyholder information.
B. Print the patient insurance information from CoPath and attach to the requisition; this is required even if the insurance fields on the form are completed.
C. Hard copies of the blank UIDL requisition are stored in the AP Documents folder.
D. Have the pathologist on the bench verify the clinical history and findings and sign where indicated.
3. Copy the completed requisition and file in AP paperwork.
4. Package the specimen according to UIDL guidelines as specified in the “Skin Biopsy for Immunofluorescence Overview” specimen packaging section.
A. Kits are located in the AP paperwork area.
B. Kits are supplied by UIDL; to order additional kits call (866) 844-2522.
Note: If the specimen is sent and will arrive on a weekend or holiday, call UIDL at (866) 844-2522 to inform them of a specimen being sent. It is not necessary to call routinely Monday through Thursday when sending a specimen.
5. Shipment: Specimens are transported via Central Delivery Service (CDS) before 2 P.M. Refer to the “Transport of Specimens to UHL/UIDL” procedure.
Note: If CDS has already picked up specimens for the day, send the specimen via FedEx using the UIDL pre-printed airbills included in the kit.
6. Record the send out in the SSO function of CoPath. Enter a return date of 30 days out so a missing report can be tracked. Pathology materials are not returned.
1. Skin Biopsy for Immunofluorescence Overview, UIDL, Iowa City, IA. http://www.medicine.uiowa.edu/uidl/
i. June 2009 L. Theisen
ii. August 2011 B. Wederquist, K. Kalish (Revised: entire document)
Interim review: August 2011 K. Kalish (Revised: II.5.)