Insulated coolers are used to transport cultures. Cultures are transported to the Microbiology Department at Cathedral Square according to the following schedule.
1. MMC-DV Site:
Monday through Friday: 0900 and 1500 shuttle via the Mercy courier
Saturday and Sunday: 0900 shuttle via the Mercy courier
Note: The Mercy courier delivers the coolers to MMC-DBQ UCL. The UCL couriers then take the coolers to Microbiology following the MMC-DBQ schedule below.
2. MMC-DBQ and Finley Sites:
Monday through Friday: 0700 and throughout the day until 1530
Saturday and Sunday: 0700 and 1100
1. Anaerobe Jar:
A. Read the indicator in the anaerobe jar and record the results on the Microbiology Daily Q.C. Log.
B. Open the anaerobe jar and remove all the plates and/or Thioglycollate tubes.
C. Place the plates in a metal can. Tape the plates into the can to avoid tipping or contamination. Place the can in the cooler for transport.
D. Place all tubes in a transport container in an upright position. Be sure the Thioglycollate tubes are securely positioned to prevent tipping and aeration. Place the transport container in a plastic bag and use a twist tie to seal the bag. Place the container in the cooler for transport.
E. Discard the used indicator and AnaeroPak.
Remove all bio-bags from the incubator and put them into the cooler.
3. CO2 Jar (Candle Jar):
A. Remove all plates from the jar and place them in a metal can.
B. Tape the plates into the can to avoid tipping or contamination.
C. Place the can in the cooler for transport.
D. Place the three QC plates into the can for transport to the Micro-CS after fresh Q.C. plates have been inoculated. Refer to the “Quality Control, Microbiology” protocol for details.
4. Blood Culture Bottles:
Place positive blood culture bottles in a specimen cup along with a Gram stained slide in a biohazard bag with a copy of the requisition.
Note: Blood cultures must be sent to the Micro-CS as soon as possible (via next routine shuttle).
5. Other Specimens:
Place all other specimens in the cooler (i.e.: Stool Culture, Ova and Parasite, etc.). Be sure the specimens are packed to prevent leakage or breakage.
Note: Because stool cultures are not plated in the laboratory, they should be sent to the Micro-CS as soon as possible (via next routine shuttle) after we receive them.
6. Tissue Specimens:
Send tissue specimens to the Micro-CS as soon as possible after arrival in the lab. Transport the specimen in a sterile urine cup containing sterile saline whenever possible. The specimen may also be sent in transport media, but this is less desirable. If the specimen is received after Micro-CS operating hours, grind the tissue with a sterile disposable tissue grinder and process accordingly. Refer to the “Primary Plating Media; Bacteriology Specimens” procedure for further details.
Place all necessary paperwork, for the cultures being sent, in the cooler for transport.
i. October 1978 M. Roth, M. Metz, E. Steiner
ii. May 1992 S. Hosch, S. Rodriguez, E. Steiner (Revised: 3.A., 4.A, and 7.; 3.B. and 8. added)
iii. February 1995 S. Hosch, S. Rodriguez, E. Steiner (Revised: I.4. note added, I.2.added)
iv. August 2002 A. Hall (Revised: combined various site protocols into one document.)
v. December 2002 R.A. Maiers (Revised: II.1.E.)
vi. April 2003 J. Wedig (Revised:I.2.)
September 2009 S. Hosch (no change)
June 2010 S. Dunn (Revised: II.4.;6.added)
May 2012 J. Klein (no changes)