Principle: | Personnel: | Specimen: | Materials: | Reagents: N.A. | Standardization: N.A. | Procedure: | Limitations: | Result Derivation: | Expected Result(s) | Quality Control: | References:
This test is performed as a definitive function to monitor the efficacy of processing, washing and concentrating Red Blood Cells (RBC) shed during surgical interventions. A cell salvage device collects and then separates anticoagulated whole blood into its individual components through centrifugation. This process continues until there is an adequate high density red cell volume. The red cell volume is cleared of contaminates by washing with one liter of isotonic saline. After washing, the clean, packed RBC is transferred to a reinfusion bag, and can be transfused into the patient.
A quantitative analysis sample should be performed on a quarter-annual basis.
2.0 ml washed RBC, obtained from the infusion bag.
1. Haemonetics Cell Saver 5 Autologous Blood Recovery System
2. 3cc ABG syringe (Marquest #021-7003487-03)
1. The Cell Saver 5 is set up and operated according to the following Mercy Medical Center Patient Care Services policies:
A. Cell Saver – Set Up and Operation – OR619
B. Autotransfusion of Washed RBC
2. A 2.0 ml sample of washed RBC is withdrawn from the reinfusion bag.
3. This sample is run on the GEM Premier 4000. Refer to the “Gem Premier 4000 Ancillary Blood Gas Analyses” procedure.
4. The hematocrit (HCT) is recorded and the report is stored in the Perfusion Office.
The resultant hematocrit may be affected by:
1. The use of manufacturer-provided preprogrammed washing procedures and operator-defined options, which allow for alteration of processing parameters.
2. The exposure of washed RBC to certain pharmocologic agents and contaminants.
3. The presence of malignancy and/or hematologic disorders.
GEM Premier printout or a transcribed copy is saved in the Perfusion Office.
1. The hematocrit of the washed RBC should be greater than 50%.
2. If the hematocrit of the washed RBC is not greater than 50%, the manufacturer will be contacted to function check the equipment and operator technique will be reviewed.
1. Cell Saver 5 Autologous Recovery System Operator’s Manual.
2. Serrick, C.J., et. al.; Quality of Red Blood Cells Using Autotransfusion devices: A Comparative Analysis. JECT 2003; 35: 28-34.
3. Shulman, Gerald, M.D.; Quality of Processed Blood for Autotransfusion. JECT 2000; 32: 11-18.
i. April 2007 R. Beecher, Perfusionist/B. McNeer, Perfusionist
April 2011 R. Beecher/L. McGovern (Revised: for Gem Premier 4000)
January 2012 R. Beecher/L. McGovern (no change)